【摘要】 本文介绍一种纯化线粒体 DNA的新方法。提取线粒体总核酸后,采用 NaCl盐析法,将线粒体大分子RNA与线粒体DNA分开,得到线粒体DNA 粗制品。再用聚乙二醇6000沉淀法进行纯化,从而得到纯的线粒体DNA。 此方法与 Sepharose 4B柱层析及氯化铯—溴化乙锭密度梯度超速离心纯化线粒体DNA的方法比较,具有经济、简便的特点,同时还可分别得到大分子线粒体RNA及小分子线粒体RNA。用此法制备的线粒体DNA易于酶切。更多还原

【Abstract】 This paper described a new method to purify mitochondrial (mt) DNA. The procedure is that: after the total mitochondrial nucleic acids were isolated, the mtRNA with high molecular weight was precipitated by 2M NaCl solution and the crude mtDNA which mixed with mtRNA of low molecular weight was obtained. Then the mtDNA was purified by PEG6000 precipitation.This method, comparing to the Sepharose 4B gel filtration and CsC1-EB gradients centrifugation, was economic, simple and rapid. It also can be used to isolate mtRNAs. The pure mtDNA obtained was easy to be cut by the restriction enzymes.更多还原