【摘要】 本文报道海南岛与苏皖地区恶性疟原虫分离株在体外连续培养中生长特征、药敏性、同工酶谱、表面抗原等方面的比较研究。海南岛两株原虫适应体外培养的速度及增殖率均明显高于苏皖两株原虫,但后者产生配子体的能力较强。体外微量药效测定显示,海南岛两株均表现对氯喹有高度抗性,而苏皖两株则均为敏感株,此外不同原虫分离株在同工酶谱及单克隆抗体间接荧光试验中亦可显示明显的株间差异。文章对恶性疟原虫种内不同虫株间生物学特性的差异进行了讨论。更多还原

【Abstract】 This paper reports some of the biological characteristics of P. falciparum isolates from different regions of China, including their growth diversity, drug sensitivity, isogenzyme variation and indirect fluorescent reaction with the monoclonal antibodies. Four cryopreserved isolates were recoyered and maintained in continuous in vitro culture by Trager-Jensen’s method. Two of them, FCC-4/HN and FCC-5/HN from Hainan, had a good adaptation to cultivation and their multiplication rate was 8-10 folds per 96 hours; the other two, Jiangsu FCC-102/JS and Anhui FCC-101/AH, showed a month or longer adaptation period before they grew stablely in culture, and had a lower multiplication rate of 4-6 folds per 96 hours. The isolates, except FCC-102/JS, adapted easily when culture medium changed from human serum to rabbit’s. In all cultures of isolates, gametocytes were found, while the gametocytogenesis of the parasites from Jiangsu and Anhui was stronger than that of the isolates from Hainan, and one of them, FCC-102/JS, maintained in culture for about 8 months, still had a high gametocyte rate of 10%.The results of 48-hour in vitro microtechnique showed: FCC-1/HN, FCC-101/AH and FCC-102/JS were sensitive to chloroquine with a mean IC50 of 46.86 ± 4.67 nmol/L; and FCC-4/HN, FCC-5/HN and Cambodian-I strain were resistant to the drug with a mean IC50 of 246.36±34.56 nmol/L.Among the isolates from the separate geographical areas, isoenzyme variation was found in polyacrylamide gel slab electrophoresis. Two types (fast and slow) of both 6PGD and GPI were detected in the isolates from Hainan and Cambodia, while only the slow bond cccured in the parasites from Jiangsu and Anhui. LDH and GDH manifested a single band in all the isolates, but the mobility of the GDH in Anhui strain FCC-101/AH was slower than that in other isolates.McAb against FCC-1/HN strain was used to demonstrate -antigenic diversity of the isolates in IFA. Most of tested McAbs reacted well with all isolates and appeared specifically fluorecent; while the antibody secreted by the hybridoma 95A4 didn’t bind the parasites of FCC-102/JS, bus reacted with other isolates.The importance of characterizing different isolates of Plasmodium falciparum has been discussed.更多还原