【摘要】 四膜虫S1是一株自接型四膜虫,根据形态特征应属梨形四膜虫复合种。本种除克隆内接合外,和其它十二种四膜虫均不接合。本种的异柠檬酸脱氢酶,四唑氧化酶,谷氨酸脱氢酶和乙酸酯酶的同功酶谱和其它十二种四膜虫相比,差异明显。此外,金亦石等(1987)所提供的S1和其它十种四膜虫的rDNA分子的四种限制性内切酶图谱也说明,S1株与除T.australis以外的其它九种的酶切图谱是显著不同的。据此,作者认为本种应是梨形四膜虫复合种中一新种,并定名为上海四膜虫。更多还原

【Abstract】 By morphological study, mating experiments and electrophoretic analysis of isozymes, as well as by study of restriction maps of rDNA molecules by Jin, et al. (p, this issue), the Sl strain of Tetrahymena collected in Shanghai and used in this laboratory for cytological studies has been proved to be a new micronucleate species of the complex species of Tetrahymena py-riformis and is named Tetrahymena shanghaiensis.This new species is a selfer species, and selfing always produces healthy offsprings which are also selfers. Carefully designed mating experiments between this species and 2 mating types of each of the 12 species hitherto established (not including the 2 new species by Simon,-et al., 1985), i.e., T. thermophila, T. sonneborni, T. tropicalis, T. pigmentosa, T. Capricornis, T. boredis, T. americanis, T. australis, T. nipissingi, T. hegewischi, T. canadensis and T. hyper-angularis, showed that conjugation did not occur between this and any other species. The composition of isozymes of isocitrate dehydrogenase (IDH), tetrazolium oxidase (TO), glutamic dehydrogenase (GDH) and acetyl esterases (ACE) of this species differed markedly from those of most of other species, with the only exceptions of the electrophoretic patterns of TO and GDH, of which, under the experimental conditions, the former was identical to that of T. nipissingi and the latter identical to that of T. sonneborni. Electrophoretic pattern of the isozymes of ACE of this species was comparable, but not identical to that of T. australis.The restriction maps of rDNA of this species are markedly similar to those of T. austra-lis. This, as well as the similarity between the ACE electrophoretic patterns of this species and T. australis, is worthy of further investigation from the phylogenetical and geographical point of view.更多还原