【摘要】 本工作观测了不同浓度棉酚(10—80μmol/L)对海胆精子鞭毛轴丝及力蛋白ATP酶的影响,发现棉酚对之具有剂量依赖方式的抑制作用。 实验对比了几种ATP酶抑制剂对轴丝ATP酶的作用,结果表明它们之间差别很大。使酶活力下降一半所需的各种抑制剂浓度分别为:钒酸钠17μmol/L;棉酚36μmol/L;槲皮酮450μmol/L,DCCD[双环已基碳化二亚胺(Dicyclohexylcarbodiimide)]1,600μmol/L;乌本苷>2,000μmol/L。 本文对棉酚的杀精机理,抑制强度及其控制生育的意义进行了讨论。更多还原

【Abstract】 Gossypol has a doserdependent (10-80 μmol/L) inhibitory effect on the axonemal and dy-nein ATPase. There are significant differences in the inhibitory potency on axonemal ATPase among several ATPase inhibitors. The dosages of these compounds calculated by 50% decrease in enzymatic activity are: sodium vanadate 17 μmol/L; gossypol 36 nmol/L; quercetin 450 μmol/L; DCCD 1,600 μmol/L and ouabain 2,000 μmol/L.The mechanism of spermicidal effect of the gossypol and its potentiality in fertility control have been discussed.更多还原