【摘要】 大鼠食管癌裸小鼠细胞株在国内外均很少见,本实验室于1986年11月开始,将体外恶性转化的大鼠食管癌细胞系(RE_25-3)接种于Swiss及NC两种裸小鼠皮下,并传代获得成功,至今已传至14代,该移植瘤具有生长稳定、接种成功率高（100％）,潜伏期短及无自发消退等特点。形态学观察:光镜下肿瘤为高分化鳞癌;电镜观察,超微结构符合高分化鳞癌的改变。流式细胞仪（FCM）的DNA分析,其DNA含量的DI值与其在体外培养的母细胞系相似,但细胞周期各期细胞的比例有明显差异。在裸小鼠体内生长的移植瘤,其S期及G₂₊M期细胞比例明显降低,说明体内生长的移植瘤其生长增殖能力较体外培养的细胞系为低,生长较慢,可能与宿主对移植瘤的作用有关。更多还原

【Abstract】 ARE 25/N nude mice model was established by subcutaneous inoculationof rat esophageal carcinoma cell line (RE 25-3). It had been maintained for more than one year through 14 passages. The tumor grewqute steadily and fast. No regression was observed. Both fight and electronic microscope observations showed that the tumor was a well diff- erentiated squamous carcinoma. The result of flow cyto metric analysis demonstrated that the DNA index was the same as that of the RE 25-3 cells cultured in vitro. Reducing of the S phase and G 2+ M phase cells and increasing of the Go/G 1 cells indicated that cell division and DNA synthesis were slowed down when cells, growhg in the nude mice.更多还原