【摘要】 本文介绍一种免疫毒素的制备方法,利用抗体大分子封闭毒素的半乳糖结合位置,减小毒素的非特异性毒性,同时,较之抗体—毒素A链结合物,对靶细胞具有更高的毒性。经放免和酶免分析证明,抗体—毒素结合物中抗体和毒素各自的特性没有改变。体外细胞毒性试验结果显示,在10~9M浓度,抗体—毒素抑制靶细胞的~3H亮氨酸参入52％,其毒性高于抗体—A链结合物的10倍,同样浓度,对非靶细胞无毒性。更多还原

【Abstract】 A method is described for linking intact ricin to antibody in a manner producing hindrance to the galactose-binding sites on the B chain of the ricin . The immunoto-xin was synthesized by reacting iodoacetybted ricin with thidated antibody.The antibody cativity of monoantibody-ricin was measured by ELISA, the results showed that the function of antibody binding to the target cells was retained. The study on the cyto-toxicity of the immunotoxin showed that it inhibited the 3H Ieucine incorporation to the target cells by 52% at a concentration of 10-9 M, it is about tenfold as potent as antibody-RTA, and the immunotoxin was not toxic at a concentration of 10-9 M to cultured normal fetal lung cell.更多还原