【摘要】 取异源八倍体小黑麦“h739”的植株和花粉植株护颖分化期的幼穗,培养在附加2mg/l2,4-D 的 MS 培养基上,接种后20天形成愈伤组织。诱导频率达98.3%。转分化培养,植株再生率为95.2%。选出了 HC-2、HC-3、HC-4、HC-5和 HC-0 5个无性繁殖系,这些系经892天的长期继代培养,植株再生率仍保持在90%以上。HC-2、HC-3经细胞学观察表明,大部分细胞为单倍体(2n=28)。组织学和电镜观察表明 HC-3系是由不同发育阶段的胚状体组成。更多还原

【Abstract】 Growing tip of allooctoploid Triticale‘h739’plants and its pollen plants at the glume dif- ferentiation stage were cultured on MS medium supplemented with 2 mg/l 2,4-D.The callus began to be formed after 20 days.The induction frequency was up to 98.3%.By transferring subcultured calli to regeneration medium plantlet regeneration frequency was 95.2%.Five so- maclones HC-2,HC-3,HC-4,HC-5 and HC-0 have been selected;their plantlet regeneration frequency remained high through long-tern subculture,being more than 90% by 892 days.Most cells in HC-2 and HC-3 were haploidy(2n=28).Histological and scanning electron micros- copy studies showed that callus of HC-3 was composed of embryoids at different developmen- tal stages.更多还原