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间接血凝法和动物法测定人血清破伤风抗体的比较

Comparison between Indirect Hemagglutination Method and Animal Inoculation Method for Determination of Anti-tetanus Antibody in Human Serum

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【作者】 周云冲王家彩杨冰

【Author】 Zhou Yunchong,Shanghai Institute of Biological Products,Ministry of Health

【机构】 卫生部上海生物制品研究所

【摘要】 本文报导用间接血凝法和小白鼠中和试验法,同时测定342份人血清破伤风抗体效价。发现两种方法的测定结果密切相关,相关系数(r)为0.88(p<0.0005)。当人血清中破伤风抗体达到间接血凝效价0.156Hu/ml 时即相当于动物法的0.01Iu/ml。认为间接血凝法快速、简便、经济,在临床及流行病学工作中,可以代替动物试验测定法。

【Abstract】 Antitetanus antibody titers in 342 human serum samples were determined simultaneously by indirect hemagglutination method and neutralization test in mice,The results obtained by these two methods were found to be closely correlated with a correlation coefficient (r) of 0.88(p<0.0005).The antitetanus antibody titer determined by animal inocylation method was 0.01 IU/ml when the titer reached 0.156 HU/ml by indirect hemagglutination method,It is considered that the indirect hemagglutination method is inexpensive,rapid and easy to perform and may take the place of animal inoculation method.

  • 【文献出处】 中国公共卫生 ,China Public Health , 编辑部邮箱 ,1987年03期
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