【摘要】 <正> 在病毒性肝炎防治工作中,HBsAg的检测至为重要。我国近几年来的HBsAg检测技术有很大进步,但目前仍有不少问题。放射免疫技术仅能在少数研究单位应用。ELISA试剂仍不理想,检测过程采用二步孵育法,费时费力,酶标记抗体消耗量大,不利于推广普更多还原

【Abstract】 A new technique for the detection of HBsAg by one step incubation ELISA was developed. Two strains of monoclonal hybridoma cell (D2H5 and H3F5,) were selected for the production of monoclonal anti-HBs, and the antibodies were used respectively for conjugation with peroxidase or for coating the solid phase in this ELISA system.The serum samples and the enzyme conjugate wc’s added to the coated wells at the same stage. After incubation at 45℃ for one hour, the wells were washed and substrate (TMB) was added for colour development. The sensitivity level of this ELISA system was up to 1 to 3 ng HBsAg/ml with P/N ratio 2.1 to 6.0.32 HBsAg negative samples were tested by this system repeatedly, no false positive result was found. 269 HBsAg carrier sera were detected parallelly by both RIA and this ELISA system by different technicians, and the results were all found to be positive. 1000 samples from the population in Guangzhou area were determined by RIA and this ELISA system by two groups of technician, the results were highly comparable. The authors considered this assay to be a technique of high sensitivity, simplicity and specificity.更多还原