【摘要】 本文报道用免疫荧光法鉴定双歧杆菌的研究成果。观察了间接免疫荧光法IIF与固相抗体吸附免疫荧光法(SPAIF)双对歧杆菌鉴定的特异性与敏感性。IIF对青春型双歧杆菌(对其本身)的最高滴度可达1:5120,而对双歧杆菌其它菌株以及其它菌种,如干酪乳杆菌、大肠杆菌、梭菌及伤寒沙门氏菌(H901w及O901w)的滴度均低于1:1280。其次,SPAIF也显示同样的特异性与敏感性。从实验结果可见,IIF与SPAIF可以作为双歧杆菌鉴定的一个手段。更多还原

【Abstract】 This paper reported the research results for identification of Bifidobac-terium with immunoflourescent technique.We have observed the specificity and sensitivity of indirect immunoflourescent method (IIF) and solid phase antibody absorbent immunofluorescent method (SPAIF).The maximum titer of fluorescent labeled antibody in IIF to Bifidobacterium adolescentis reached 1:5120,while to other strain of B.adolescentis and other species of intestine such as Lactobacillus Casei,E.coli,Clostridium,S.typhi H901w and O.typhi 090lw as low as 1:1280 with IIF.The same specificificity and sensitivity was exhibited in above bacteria with SPAIF.According to these experimental results,it could be believed that these methods may be one means for identification of Bifidobacterium,if the fraction of common antigen between the Bifidobacterium and other intestinal bacteria was cemoved.更多还原