【摘要】 本文着重研究广西普通野生稻在离体条件下,诱导率,分化率与原分布地、形态类型及其优良特性的关系。试验共采用了28个编号,接种了近63000个花药,获得了1447块愈伤组织。野生与半野生类型的诱导率分别为0-15.31%和0-8.5%。18个编号出了白苗529株,4个编号出了绿菌22株。野生与半野生类型白化苗分化率为18.75-71.43%和9.09-58.82%;绿苗分化率为2.56-8.22%和5.88-13.33%。鉴定了部分编号的再生植株,染色体数n=12,2n=24。分化绿苗的材料(编号1、10、19、26)具有抗稻瘟病、高蛋白质含量、耐寒性强和抗白叶枯病的特性,这些材料为水稻育种提供了宝贵的遗传资源。更多还原

【Abstract】 The anther culture of common wild rice with fine agronomical traits in the Guangxi Zhuang Autonomous Region was studied. In the in vitro anther cultue, the study was focused on the correlation between the frequency of induction and differentiation and the correlation between morphological types and original distribution regions. About 63000 anther of 28 strains were inoculated and 1447 pecies of calluses were obtained. The induction frequencies of wild and semi-natural types were 0-15.31% and 0-8.5% respectively. The results indicated that 18 strains differentiated into 529 albino plantlets, and 4 strains differentiated into 22 green plantlets. Albino plantlets differentiation frequencies of wild and semi-natural types were 18.75-71.43% and 9.09-58.82% respectively, and those of green plantlets were 2.56-8.22% and 5.88-13.33% respectively.The chromosome observation of root-tip in a part of acquired plantlets showed n=12, 2n=24, Some green plantlets (No. 1, 10, 19, 26) exhibited rice blast resistance, high protein contents, cold resistance and bacterial blight resistance. There can provided valuable genetics resources for rice breeding.更多还原