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电脑在青霉素酰化酶及其固定化酶动力学米氏常数测定中的应用

THE APPLICATION OF COMPUTER IN THE MEA1SUREMEINT OF KINETICS CONSTANT (KM) FOR PENICILLIN ACYLASE AND ITS IMMOBILIZID INZYME

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【作者】 蒋昱戎明阳王刚黄琦

【Author】 Jiang Yu, Rong Ming-yong, Wang Gang Huang Qi(Dept. of Biosynthetic Drugs, School of Pharmacy; Microcomputer Group of Equipment Division, Shanghai Medical University, Shanghai)

【机构】 上海医科大学上海医科大学 上海上海上海

【摘要】 本文测定青霉素酰化酶的动力学米氏常数Km值和固定化酶不同颗粒度的表观Km值,应用电脑计算、自动作图,比常规的作图法误差小,既精确而又简便。这是电脑技术在生化领域研究中应用的一个实例。

【Abstract】 The present paper reported the measurements and comparisons of Kinetics constant (Km) for penicillin acylase and its immobilized enzyme manifestation Km of various granularity. The computer calculation and auto construction which works more accurately than the routine construction, were employed. This is an example of the application computer technique on the studies of biochemical field.

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