【摘要】 本文报道反相高效液相色谱法定量测定抗生素2488和日本进口柱晶白霉素组分含量的方法。色谱柱是MICROBORE C18,移动相是甲醇-1/15M乙酸钠缓冲液（54/46）,紫外229nm波长检测。本法可使柱晶白霉素A₁,A₃～A₉等八个组分和两个未知成分获得良好分离。定量结果表明,抗生素2488的六个有效成分A₁、A₃～A₇的总量与进口柱晶白霉素接近。更多还原

【Abstract】 This paper dealt with a quantitative method for determination of Antiliotic No.2488（home-made Leucomycin） and Leucomycin （made in Japan） by reversed-ph-ase high performance liquid chromatography. The chromatography separation was performed with MICROBORE C₁₈ column and mobile phase consisting of ,a methanol -1/15 M sodium acetate buffer （pH 4.9）,at a ratio of 54/46 v/v. The detective wavelength 229nm was used.By using this method, leucomycin A₁, A₃^A₉ and two unknown components were separated. The results have shown th .t six effective components A₁, A₃^A₇ of antibiotic No. 2488 accounted for 80 to 95% to the total content which was comparable with the imported leucomycin.更多还原