【摘要】 本文报道用五种不同的包被抗体(抗-IgM,Dako 抗μ、Cappel 抗μ,本所抗μ和抗μ链单克隆抗体).经 IgM 捕捉免疫测定法对104份乙型肝炎血清学标志不同的血清和36份正常人血清作了 IgM 抗-HBc 的对比检测,结果揭示本所自制的抗μ具有较高的特异性。用本所抗μ仅在 HBeAg 阳性和 HBsAg 单独阳性血清标本中测到IgM 抗-HBc(与其他几种包被抗体无区别);而在抗-HBe 阳性的血清标本中检出率却很低,与用抗μ链单克隆抗体检测结果相似。其结果与其他几种包被抗体有着明显的差异.更多还原

【Abstract】 A comparative determination of IgM anti-HBc was done by means of IgM-capture immunoassays in 104 sera of various serologic markers of hepatitis B and 36 NHS with the application of five differentlycoated antibodies,including anti-IgM,anti-μ(Dako),anti-μ(Cappel),anti -μ.(prepared by the authors)and monoclonal IgM antibody.The result exhi bits that anti-μ prepared by the authors has a greater viral specificity than others.Since anti-HBc IgM is detectable only in HBeAg positive and HBsAg singly positive sera,it shows no difference from others. However,the detection rate of anti-HBc IgM is very low in anti-HBe positive serum specimens and thus the results are simillar to those done with monoclonal IgM antibody.The detection rates differ apparently between the selfmade anti-li and the others.更多还原