【摘要】 本文研制了¹²⁵I标记的PGI₂稳定的代谢产物6-酮-PGF_1αRIA试剂盒。以碳化二亚胺法将6-酮-PGF_1α与BSA联接免疫家兔制备抗血清,其效价为1:32000,平衡常数为6.26×10⁹L/mol,与其他前列腺素的相对百分交叉反应<1%。用氯胺T法制备¹²⁵I-组织胺-6-酮-PGF_1α标记物,比放射性活度>14.8MBq/μg（400μCi/μg）,最高结合率>80%,二月内无脱碘现象。放免测定按常规法,最低检出值为2.2pg/管,检出率为97.2～102.3%,精密度为6.62%,批内和批间CJ.V.分别为7.17、8.41%,Cerceo“效点系统”评分为31分,属优良。直接测定健康成人血浆6-酮-PGFl_1α浓度为22.9±6.3pg/mL。更多还原

【Abstract】 A radioimmunoassay kit for 6-keto~PGFltI was prepared in which 12B I?labeled histamine derivative was used. The antiserum was raised by immunizing rabbits with 6-keto-PGFltl-BSA conjugate. Its specificity was satisfactory, presenting minor crossreactions (<J%) with different prostaglandins, and its titer was 1:32 000.The 12 BI-histamine-6-keto-PGF,a was prepared using the chloramine-T procedure,the specific activity was over 14.8MBq/ng(400nCi/Vg). The minimal detectable limit was 2.2pg per tube. The recovery rate was 97.2~ 102.3%.The accuracy was 6.62%, and the intra- and inter-assay coefficients, of variation were 7.17% and 8.41% respectively. The normal plasma 6-keto-PGFlct value was 22.9?.3 pg/mL (J+ SD, n = 20) .更多还原