【摘要】 电子显微镜观察发现,急型克山病人心肌线粒体内出现两种电子密度内含物,一种是无定形的,另一种是针状高电子密度的,这两种内含物的出现是心肌细胞不可逆损伤的重要标志,经电子染色,X 射线显微分析,以及次氯酸钾消化三种方法鉴定结果证明,中等电子密度无定形内含物是线粒体蛋白质的变性凝聚,针状高电子密度内含物是含钙物质。本文对这两类内含物出现的条件作了分析和讨论。更多还原

【Abstract】 The intramitochondrial inclusions occurring in myocardial cells of theacute Keshan disease have been observed by the electron microscopy.Wefound that these inclusions were classified according to their feature intotwo categories:1)moderately electron-dense amorphous,floculant inclusion,2)high electron-dense spicular inclusion.All of these ultrastructural fea-tures indicated that the myocardial cells had been irreversible damaged.In our experiment,three ways were used,which were the electron stain-ing,the X-ray microanalysis and digestion with potassium hypochlorite,so toidentify the chemical characteristic of the intramitochondrial inclusions.The results above indicate that the composition of moderately electron-dense amorphous,floculant inclusions is denatural materials of proteins in mitochondria and that the composition of high electron-dense spicular inclu-sions is material of mitochondrial calcification.In addition,the cause and process of mitochondrial calcification werediscussed.更多还原