【摘要】 用ELISA和RNA PAGE银染法检测了上海市第一人民医院新生儿病房及隔壁婴儿病房8份急性腹泻粪便标本;3份粪标本呈轮状病毒特异抗原阳性,3份粪标本能提供RNA进行电泳分析。6份病人恢复期血清抗体效价均>1:1280,较正常配对组的抗体水平高4倍以上,说明新生儿病房及隔壁婴儿病房急性腹泻流行为轮状病毒感染所引起。分析其RNA迁移图谱,均为电泳型2L,第3电泳亚型。经混合电泳显示3例RNA无差异,提示病人感染来自同一传染源;并探讨了轮状病毒传播的可能途径,为今后改进医疗质量提供了资料。更多还原

【Abstract】 Eight stool samples of acute diarrhea obtained from the new-born baby wards, the Shanghai First people’s Hospital were investigated by ELISA and RNA PAGE silver-staining method. Three samples are rotavirus specific-antigen positive, and three samples could offer enough RNA to analyse. Antibody titer of convalescent serum of six patients were higher than 1280, four times as high as that of control groups The result showed that the epidemic of acute new-born baby diarrhea was caused by rotavirus, and the migration petterns of RNA genome were all electrophorotype 2L, belonging to 3 subtypes. RNA patterns were similar to each other by co-eletrophcresis, and the infection of rotavirus probably came from the same origin. The possible pathway of communication of rotavirus was discussed, and the methods were offered to improved the quality in medical care.更多还原