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甘胆酸放射免疫分析的几个问题
SOME PROBLEMS CONCERNING RIA OF CHOLYLGLYCINE
【摘要】 本文讨论了甘胆酸组胺(CGH)的碘化标记,单125I-CGH免疫活性(B0/T=70%)>双125I-CGH免疫活性(B0/T=45%)。对甘胆酸抗血清质量(亲和常数K,交叉反应率和滴度)作了研究。从logit-log线性计算结果指出,抗血清质量影响着放免分析的测定。保护蛋白影响着抗血清结合率。CG标准采用特殊的去激素血清配制。
【Abstract】 In this paper iodine labelling of cholylglycylhistamine (CGH) is discussed. Theimmunoactivity of monoiodo-CGH (B0/T=70%) is greater than that of diiodo-CGH(B0/T=45%). Antiserum quality of cholylglycine(CG) (affinity constant K crossreactivity and titer) is studied. The relation between the quality and radioimmun-oassay is shown by logit-log plot. Antiserum binding is effected by protectionproteins. The CG standard is prepared with special serum.
【关键词】 放射免疫分析;
碘标记CGH;
抗血清;
保护蛋白;
【Key words】 Radioimmunoassay; Iodine labelled CGH; Antiserum; Protection protein;
【Key words】 Radioimmunoassay; Iodine labelled CGH; Antiserum; Protection protein;
- 【文献出处】 原子能科学技术 ,Atomic Energy Science and Technology , 编辑部邮箱 ,1987年02期
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