【摘要】 用薄层色谱法考察了NaOH浓度对分离的影响,选用了含0.3 mol/L NaOH的0.5%CMC-Na溶液调和制板,以甲醇—乙酸乙酯—环己烷(10:45:45)为展开剂可将螺旋霉素完全分离,以甲醇—乙酸乙酯—环己烷(8:46:46)为展开剂可将乙酰螺旋霉素基本分离。用CS-910薄层扫描仪对样品进行了定量分析。更多还原

【Abstract】 Thin-layer chromatography was used for separation of spiramycin (SPM) and acetylspiramycin (Ac-SPM). 0.3 mol/L NaOH in 0.5% CMC-Na solution was selected to spread plates with silica gel (3.3:1), methanol—ethyl acetatecyclohexane (10:45:45) and (8:46:46) were employed for separating SPM and AcSPM respectively. It has been found that the content of each component in SPM made in China is different from that made in Japan, namely, in the former SPM Ⅱ, Ⅲ are the chief components, but in the latter SPM, SPM Ⅰ is the chief one. Moreover, in Ac-SPM made in China, diacetylspiramycin Ⅱ, Ⅲ are dominant and in Ac-SPM made in Japan, di- and monoacetylspiramycin Ⅱ are the principal components.更多还原