【摘要】 <正> 花叶芋是室内观叶植物,原产热带美洲亚马逊河流域。国内外在花叶芋的试管繁殖上已见若干报道,本文着重研究了花叶芋的成苗速度及液体培养的效果。更多还原

【Abstract】 This report describes the rapid propagation of Caladium hortulanum and C. bicotor (three cultivars) by using tissue culture. First of all, take the young leaf and petiole as explants and transferred on Murashige and Skoog’s agar medium supplemented with benzyladeninc 2 mg/1 and naphthalene acitc acid 0.6-1 mg/1, most of them conduced callus on the cut surface within 2-3 weeks. Without subculturing 5th weeks it was discovered much globularity and smooth embryoids that differetiated on the surface of callus. They continued to develop and grow heart-shaped leaflet, them rooted, formed complete regenerate plantlets. Results showed that the light and darkness condititions didn’t effect the callus conduct and cmbryogenesis. The second period we suggested by method of static culture in liquid medium (MS + BA 1, omitted agar) which enabled the proliferation speed of the clonal plantlets of Caladium to 3- 4 times per month, and estimate the maltiplication rate acheved about 3.4×l06 times per yeay.更多还原