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应用酶联免疫吸附法检测血清和泪液中抗衣原体IgG抗体
Enzyme-Linked Immunosorbent Assay for IgG Antibody to Chlamydia Trachomatis in Human Sera and Tears
【摘要】 <正> 沙眼衣原体是一种细胞内寄生体,它可引起人类不同群体及人体不同器官的感染。在我国,尽管沙眼患者已明显减少,但在广大农村和一些中、小城市中,沙眼仍有着较高的患病率并是致盲的重要原因之一。同时眼科医疗工作中也有沙眼的鉴别诊断问题。另外,在衣原体感染与人的免疫反应的关系上,还存在许多
【Abstract】 Determination of specific antichlamydial trachomatis antibody in the human serum and tears by enzyme-linked immunosorbent assay (ELISA) for IgG antibody to Chlamydia trachomatis in human sera and tears is reported. The object included students with trachoma, patients with conjunctivitis, and healthy individuals. IgG antibody to Chlamydia trachomatis was positive in over 80% in both the serum and the tears from the students with trachoma; the rate of consistency between the two reached 84.8%. The titers of IgG antibody in tears were higher than those in the serum. No significant difference was found between the contents of specific IgG antibody against Chlamydia trachomatis in the serum and the tears of patients with acute or chronic conjunctivitis and those of the healthy individuals. These results show that ELISA is a simple, sensitive and specific method of detecting and determining antichlamydial trachomatis antibodies.
- 【文献出处】 眼科研究 ,Chinese Ophthalmic Research , 编辑部邮箱 ,1987年03期
- 【被引频次】1
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