【摘要】 本文研究了二种由刺参体壁提取的硫酸多糖SJAMP和HLAMP对凝血酶和内皮细胞相互作用的影响。凝血酶可以显著增加正常人脐静脉内皮细胞分泌von Willebrand因子和前列环素（PGI₂）的功能。后者利用放射免疫法测定其稳定的代谢产物6-酮-PGF₁α。经SJAMP、HLAMP或肝素预先保温,可以抑制凝血酶诱导的内皮细胞的上述分泌功能,而且这种抑制作用与SJAMP、HLAMP或肝素的浓度相关。更多还原

【Abstract】 SJAMP and HLAMP are glucose-containing acidic mucopolysacc-harides isolated from sea cucumbers, Stichopus Japonicus and Holot-huria Lecospilota respectively. Their effects on the interaction of thrombin with endothelial cells were studied. Human vascular endothelial cells isolated from umbilical veins treated with 0.1% collagenasc were cultured with MEM medium. The contents of von Willebrand factor (vWF) and 6-keto-PGFla, stable hydrolysis product of PGI,, in the culture medium were measured with ELISA and immunoradioassay technique. The addition of thrombin to confluent monolayer endothelial cells resulted in a significant increase in the release of vWF and 6 ~ keto-PGFla in the culture medium. When heparin,SJAMP or HLAMP was added, no significant amount of vWF or 6~keto-PGFla could be detected in the medium .Whereas preincubation of thrombin with heparin, SJAMP or HLAMP respectively inhibited the thrombin-induced vWF and 6-keto-PGF 10 release and this effect was well correlated with the dose of heparin, SJAMP or HLAMP, indicating that SJAMP, HLAMP as well as heparin had inhibitory effect on the interaction of thrombin with endothelial cells更多还原