【摘要】 取500头怀孕母牛的血,制成血清后,分别与随机选择的40头牛的淋巴细胞反应,方法采用国际标准微量淋巴细胞毒方法,筛选得到274份阳性血清。将这些阳性血清再用包括5个家系的60头牛的淋巴细胞进行细胞毒试验,数据经聚类分析后,得到了十三组高度相关的血清,并作出了血清图。这十三组血清图被初步命名为BoLA—W1—W13。其特异性将与国际标准BoLA进行核对。本文进一步探讨了中国黑白花奶牛的BoLA的抗原分布及应用价值。更多还原

【Abstract】 Serum samples drawn from 500 pregnant Black and White cattle were screened against a panel of 40 random of lymphocytes and produced 274 cytotoxic antisera by international standard microlymphocytotoxic method. Then, these antisera were tested against lymphocyte samples from 60 cattle belonging to 5 different sire families . Thirteen lymphocyte antigens were identified with the aid of a computer program according to their reaction patterns and designated BoLA-W to W13. The serograph is given in this paper. A comparison test of these results with international BoLA designation should be made. The antigen distribution of the BoLA and the value of its application to animal production in China were also discussed.更多还原