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奶牛胚胎切割移植试验报告

A REPORT OF THE PRELIMINARY STUDIES ON EMBRYO CUTTING AND TRANSFERING IN DAIRY COWS

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【作者】 窦忠英樊敬庄张致民胡庆义张晓红

【Author】 Dou Zhongying Fan Jingzhuang (Department of Veterinary,the Northwestern Agricultural University)Zhang Zhimin Hu Qingyi Zhang Xiaohong (Sanyuan County,Shaanxi Province)

【机构】 西北农业大学兽医系西北农业大学兽医系陕西省三原县陵前乡奶牛胚胎移植站

【摘要】 以裸胚切割和透明带内切割两种方法,切割31枚奶牛早期胚胎,一分为二。将41枚半胚(其中38枚成对)分别移植给26头受体牛,6头移植怀孕,成功率为23.1%,移植有透明带的半胚和无透明带的裸半胚怀孕成功率都为25%。2半胚在同一透明带内(透明带内切割)的怀孕成功率为40%,而将裸半胚重新装入空透明带内的移植5头(受体全为黑白花奶牛),均未怀孕。桑椹胚分割成半胚移植怀孕成功率为21.1%,而早期囊胚则为28.6%。

【Abstract】 Each of thirty-one early developing cow embryos was cut into two parts byusing the methods of bare embryo cutting and internal zona pellucid cutting. Forty-one half-embryos(38 half-embryos were paired)were transfered into 26 recitients,of which 6 recipients succeeded in gestation with the success fate of 23.1%.But the success rate of the transfers of both the half-embryos with zona Pellucid and the bare half-embryos without it was 25%.When two half-embryos(internal zona pellucid cutting)in the same zona pellucidwere transfered,40% of success rate in gestation was obtained,but the 5 recipients (all Holstein-Friesian)which were transfered with bare half-embryos put into the empty zona pellucid again,failed in gestation.When the morula cut into half- embryos were transfered,the success rate was 21.1% while that of the early developing blastocysts came to 28.6%.

  • 【文献出处】 西北农林科技大学学报(自然科学版) ,Journal of Northwest Sci-tech University of Agriculture and Forestry(Natural Science Edition) , 编辑部邮箱 ,1987年03期
  • 【被引频次】16
  • 【下载频次】61
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