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我国东方铃蟾皮肤中C-12-h肽的分离与鉴定

Isolation and Identification of C-12-h Peptide from the Skin of Chinese Bombina Orientalis

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【作者】 陈枫赵轶千

【Author】 Chen FengDepartment of Internal Medicine, Tongji Hospital, Tongji Medical University, WuhanZhao YiqianDepartment of Physiology, Faculty of Basic Medical Sciences, Tongji Medical University, Wuhan

【机构】 同济医科大学附属同济医院内科同济医科大学基础医学部生理学教研室

【摘要】 东方铃蟾(Bombina orientalis)新鲜皮肤的甲醇提取液,可引起大鼠离体平滑肌收缩。此活性粗提液经碱性氧化铝和葡聚糖凝胶G-15柱两次层析分离,所获活性C-12组分,可在糜蛋白酶水解后失活,但其活性不被赛庚啶[2×10-6]完全拮抗。用反相高效液相色谱技术(RP-HPLC)分离C-12,见C-12-h吠与铃蟾肽(Bombesim,BBS)的出峰时间相同,且有相同的氨基酸组成。结果表明,C-12-h肽极可能就是铃蟾肽。

【Abstract】 Methanol extracts of the fresh skin of Chinese amphibian, Bombina orientalis, caused contraction of the smooth muscles of Wistar rats in vitro. The active extracts were isolated twice by column chromatography of alkaline alumina and sephadex G-15, so that a bioactive eluate C-12 was obtained. The bioactivity of C-12 was inactivated by hydrolysis with chymotrypsin, but not completely, antagonized by cyproheptadine. C-12 was further isolated by reverse phase-high performance liquid chromatography (RP-HPLC). The elution time of C-12-h peak corresponded to that of bombesin standard and their bioactive peak was identical as well. The peaks of the two peptides on RP-HPLC graphs were found to overlap each other. Furthermore, it was revealed that both psptides had the same amino acid composition. The above experimental results suggest that C-12-h peptide is most probably bombesin.

【关键词】 东方铃蟾铃蟾肽分离生物测定
【Key words】 Bombina orientalisbombesinisolationbiological assay
  • 【文献出处】 同济医科大学学报 ,Journal of Tongji Medical University , 编辑部邮箱 ,1987年06期
  • 【下载频次】44
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