【摘要】 家兔从妊娠第1天始,每天经口给予炔诺酮肟4mg/kg,连续3天,第9天处死。取卵巢作HE、ACP、ALP、SDH及3β-HSD染色,部分卵巢作超微结构观察。结果表明:炔诺酮肟呈明显的抗着床作用,卵巢黄体平坦,缩小。光镜观察,黄体细胞呈较明显的颗粒变性及空泡变性。组化染色,ACP活性增强(P<0.01)。3β-HSD活性减弱(P<0.01),ALP及SDH活性无明变化(P>0.05)。超微结构可见,黄体细胞胞质中滑面内质网明显减少,脂滴聚集,溶酶体数量增多,形状多样,脂质空泡化及类似髓鞘样结构等。提示由于药物抑制了卵巢3β-HSD活性,使卵巢激素生物合成受阻;此外,ACP活性增加,提示溶酶体通透性增加。更多还原

【Abstract】 The rabbits were treated by oral administration for 3 days of 4mg/ kg/day NETO after successful coitus and sacrificed on the 9th day. The ovaries were dissected for histological examination after being stained by HE, ACP, ALP, SDH and 3β-HSD. Parts of the ovaries were also studied by ultrastructural examination. It was found that NETO showed obvious anti-implantation effect. Cor- pora lutea of ovaries became flattened and shrunk. Under light-microscopic observation, obvious vacular and granular degeneration appeared in luteal cells. In histochemical observation, the activity of ACP increased, 3β-HSD decreased and ALP and SDH remained unchanged. Under the electron microscope it could be seen that smooth eedoplasmic reticula decreased, lipid droplets accumulated; lysosomes increased, and myelinbid-like bodies and vacular lipid droplets appeared in luteal cells.From the above it may be conclmded that the biosynthesis of steroid hormone in ovary was blocked due to the inhibition of 3β-HSD activity. In addition, activity of ACP increased, suggesting an increase in lysosome permeability.更多还原