【摘要】 用突变株AS110进行培养条件考察,在适宜的条件下,该菌株可产L-异亮氨酸16.4mg/ml,其他氨基酸含量均低于0.5mg/ml。较高浓度的尿素对菌体生长和L-异亮氨酸的产生均有抑制作用。发酵产品经红外吸收光谱,比旋光测定,纸层析和生物鉴定证明是L-异亮氨酸。更多还原

【Abstract】 Cultural conditions ot mutant AS110 were mvestigated. Under suitable conditions,the strain could produce L-isoleucine(16.4mg/ml) and other amino acids (<0.5mg/ml). Urea showed an inhibitory effect cn the L-isoleucine production and growth of the mutant.The fermentation product was identified as L-isoleucine by infrared absorption spectrum, paper chromatography, specific rotation and bioassay.更多还原