【摘要】 本文用双参数FCM技术,对同一个细胞的DNA和RNA含量进行相关测量,比较了ACM B对小鼠L₁₂₁₀白血病细胞周期和RNA含量的影响.结果发现在一次给药后8小时可导致早、中期S的积累,并抑制S期细胞的DNA合成;到24小时DNA合成恢复正常,并进入G₂期,但由于G₂期细胞进入M期受阻,造成G₂期细胞的积累,这时被阻断在G₂期的细胞RNA含量显著增加,形成正不平衡生长,而给药剂量较大的实验组(1/1.5LD₅₀)S期细胞的RNA含量不随着DNA含量的增加而增加,形成负不平衡生长,ACM A和ACM B对体内Li₂₁₀细胞周期作用相同.更多还原

【Abstract】 The effect of ACM A and ACM B on cell cycle progression and RNA contents of L1210 cells were compared in vivo.By using dual parameters flow cytometer to measure cellular DNA and RNA contents simultaneously, early and middle S cells were accumulated 8 hr after treatment with 1/2,5 or 1/4.0 LD50 of ACM A or ACM B, this showedthat the DNA synthesis rate was decreased in the early and mid S phase cells. The DNA synthesis inhibition was reversible, so S cellls entered to G2 phase and caused accumulation of G2 cells due to the progression of G2 to M was blocked or slowed down by 24 hr treatment. At the same time, the RNA content of cells blocked in G2 phase were increased predominately and caused the positive unbl-ance growth. In contrast, the RNA content of S phase cell was not increased concomitantly with the increment of DNA content and caused the negative unbalance growth.In cluclusion, the effect of ACM A and ACN B on the cell cycle progression of L1210 cells in vitro or in vivo is almost the same, whereas the inhibition of RNA synthesis of L1210 cell in vivo are recovered in G2 and G1 cells but not in the S phase cells treated with ACM A or B by 24 hr.更多还原