【摘要】 我们首次观察分析了竹红菌甲素对CHO细胞的可见光(碘(钅马)灯:全光、320-460nm、350-490nm)动力敏化致死作用,并与紫外线照射的作用作了比较.竹红菌甲素光动力敏化作用所造成的细胞亚致死和潜在致死损伤较不易被修复(或累积效应较不明显).我们认为该药物的光敏作用的“靶”主要是细胞膜.更多还原

【Abstract】 Inactivation of CHO cells with visible light (iodine-tungsten lamp: pan-light 320-460nm and 350-490nm) exposure in the presence of HA has been studied, and compared with the effect by UV exposure.Wavelength involved for photosensitiza-tion of HA is closely related to absorption spectra of HA. HA’s ability of photo-sensitization at the pan-light and 460-490nm corresponds to 72% (the ratio of Do values.83/116) of UV action, when the concentration of HA is 5 μg/ml.At lower fluence,however, the effect of photosensitization(the value of quasi-threshold flu-ence Dq is lower) of HA for CHO cells is stronger than that of UV.Analysis of the survival curve parameters shows that the repair of sublethal damages and potential lethal damages of the cells by photodynamic sensitization of HA is more difficult than that induced by UV (i.e. the accumulative effects of HA’s photo-sensitization is not obvious) . Also, it is suggested that the main target of the HA’s photosensitization be cell mambrane.更多还原