【摘要】 恶性肿瘤细胞或粘着于基膜以及在一定的基质上增殖对于肿瘤的浸润转移至关重要。层粘连蛋白(1aminin,LN)是基膜所特有的非胶原糖蛋白。我们研究了LN在肿瘤细胞粘着、铺展及增殖中的作用。通过测定粘着细胞所释放的LDH活性定量分析细胞粘着率。LN可显著增加体外培养的小鼠S180-V肉瘤及B16-MBK黑色素瘤细胞在玻璃及Ⅳ型胶原基质上粘着及铺展。纤维粘连蛋白(fibronectin,FN)亦有类似作用。而非糖蛋白(牛血清清蛋白)或其他糖蛋白(鸡卵清清蛋白或免疫球蛋白)均无类似作用。此外,LN或FN抗体可分别抑制细胞粘着于LN或FN。这些结果表明LN或FN促细胞粘着作用是专一性的。扫描电镜观察表明,粘着在LN敷盖的玻璃表面的瘤细胞呈多突扁平形、膜皱襞及微绒毛十分稀少,而粘着在裸露的玻璃表面者为球形,膜皱襞及微绒毛甚多。再者,~3H-TdR向粘着于LN基质上的瘤细胞群的参入量显著高于粘着在裸露玻璃面者。更多还原

【Abstract】 The attachment to basement membranes and the proliferation on definded matrices of cancer cells are of fundamental importance in the processes of invasion and metastasis. We studied the effects of laminin on cancer cell attachment, spread, and 3H-TdR incorporation. The attachment of cells was quantitated by measuring thier LDH activity. Laminin markedly stimulated the attachment and spread of mouse S180-V sarcoma cells and B16-MBK melanoma cells in culture on solid support. Fibronectin but not other glycoproteins, such as egg albumin or immunoglobulin, or non-glycoprotein, such as bovine serum albumin, showed similar stimulation effect on cancer cell attachment. Furthermore, cell attachment to laminin or fibronectin was specifically inhibited by antibody against laminin or fibronectin respectively. These results indicated that the role of laminin was specific. The surface of attached cells was observed under scanning electron microscope. Cancer cells attached to the surface of bare glass were round shaped with numerous ruffles and microvilli. In contrast, those attached to the laminin-coated glass surface appeared polygonal and flat in mophology with fewer ruffles and microvilli. In addition, the incorporation of 3H-TdR into cancer cells attached to the laminin matrices was substantially increased. The role of laminin in the invision and metastasis of cancer cells is discussed.更多还原