【摘要】 用中国北方草原地区的季节性冬眠动物达乌尔黄鼠,经1981和1984两年的工作,重复了Dawe(1969)注射冷藏的冬眠动物血清,诱发夏季活泼黄鼠冬眠的原始实验。实验成功地实现了在非冬眠季节诱发达乌尔黄鼠冬眠,发现禁食在人工诱发冬眠中起重要作用,却不能证实血源性冬眠触发物(HIT)的存在。更多还原

【Abstract】 In an attempt to confirm E’awe-Spurrier ’s original report of blood borne hibernation induction trigger (HIT), an experiment was designed on a Chinese seasonal hibernator, Citellus dauricus.26 animals were divided into 3 groups for transfusion, group H (10 squirrels) ,was transfused with preserved sera from deeply hibernating animals in winter; group A (9 squirrels), with preserved sera from active animals in spring; and group S (7 squirrels), with 0.9% saline. After transfusions, the animals were kept in individual cages and transfered to cold dark room at 5 ℃ between May 7 and July 10. Rat chow, sun-flower seeds and fresh cabbage were supplied and daily records of each animal’s Tb and state of activities were kept. At the beginning of 5th week, supplies of seeds and chow Were reduced gradually and the animals were completely fasted after the 45th day.As a result, three kinds of responses occured in animals of all the three groups. 10 animals (5H, 4A, 1S) entered hibernation cycles after 2 weeks of restriction of food, 9 animals (3H, 3A, 3S) remained euthermic for 1-2 weeks following the complete fast and died, and 7 animals (2H, 2A, 3S) became deep hypo thermic but never aroused spontaneously and died 10-20 days later. It is interesting to note that the number of animals that show normal hibernation bouts are approximately the same in the test group H (5 out of 10) and control group A (4 out of 9).Preliminary conclusions drawn from the experiment were: 1) Summer hibernation was successfully induced by restriction of food in dark cold room in a seasonal hibernator,Citellus dauricus. 2)Seasonal hibernating species has it’s own pattern of circannual rhythm of body temperature, not all of the individuals may be induced into hibernation in nonhibernating season. 3) The data provided no satisfactory evidence for the existence of HIT.更多还原