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高效液相色谱法测定精氨酸催产素及其酶催化降解产物

Determination of Arginine-Vasotocin (AVT) and the AVT 1-8 Flaoment from Digestion of AVT by High Performance Liquid Chromatography

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【作者】 练鸿振张文彬王新昌刘斌郑集

【Author】 Lian H.ngchen.Zhang Wenlin Centor of Materials Ana-lysis. Nanjing University, Wang Xinchang,Liu Sin and Zheag Ji, Dip ar tment of Biochemistry,Nanjing University

【机构】 南京大学现代分析中心南京大学生物化学系南京大学生物化学系

【摘要】 <正> 精氨酸催产素(Arginine-Vasocotin,AVT)广泛存在于最原始的脊椎动物脑垂体

【Abstract】 This paper presents the determination of Arginine-Vasotocin (AVT) and the AVT1-8 fragment obtained from digestion of AVT by toad brain synaptic membranes with HPLC. and the proteolytic conversion of AVT. AVT and AVT1-8 are separated on a reversed phase ODS column with NH4Ac and HAc in CH3OH as mobile phase, detected by UV monitor at 230nm. This method is simple and efficient for the research or proteolytic conversion mechanism of active pep-tide, as well as for general kinetics studies on other chmical reaction.

  • 【文献出处】 色谱 ,Chinese Journal of Chromatography , 编辑部邮箱 ,1987年05期
  • 【下载频次】66
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