【摘要】 东方铃蟾鲜皮的甲醇提取液,可引起大鼠离体平滑肌的收缩。此液经碱性氧化铝柱层析分离,其80%乙醇洗脱的 C 组分显示生物活性。C 组分再经葡聚糖凝胶 G-15柱分离,其活性较强的早期洗脱组分 C₁₂,可被糜蛋白酶水解灭活,但其活性并不被5-HT 拮抗剂赛庚啶[2×10^-6mol/L]完全拮抗。继用反相高效液相色谱（RP-HPLC）分析,见分离出的 C_12h峰与铃蟾肽^*（Bombesin,BBS）的出峰时间相同,且具相同的氨基酸组成。上述实验结果提示,C_12h肽可能就是铃蟾肽。更多还原

【Abstract】 Methanol extracts of the fresh skin of China amphibians （Bombina orientalis）caused contraction of the smooth muscles of rat in vitro.The crude extracts wereisolated by column chromatography of alkaline alumina,and a bioactive fractionwas obtained from the 80% ethanol eluate.Submitting the fraction on SephadexG-15 column,we found that the early eluate C_-12,showing more bioactivity,was inactivated by hydrolysis with chymotrypsin,but not completely antagonizedby cyproheptadine.C_-12was further analyzed by reversed phase-high perfor-mance liquid chromatography （RP-HPLC）.The elution time of C_-12-h peak wasthe same as that of bombesin,and these two peptides had the same amino acid com-position.These experimental results suggest that C_-12-h peptide is most pro-bably bombesin.更多还原