【摘要】 对日本血吸虫病可溶性虫、卵抗原(Schistosoma japonicun soluble egg antigen,简称SEA)进行了检测,结果表明SEA中的糖蛋白组分具有较高的免疫活性。提取相应的抗SEA糖蛋白组分抗体,采用酶联免疫吸附法(ELISA)检测SEA抗原及其糖蛋白组分,与抗粗SEA抗体相比,敏感度提高100～1,000倍,且具有较好的重复性。更多还原

【Abstract】 This paper reports the following studies related to circulating Schistosoma japonicum soluble egg antigen (SEA): (1) purification of glycoprotein and hydrophobic glycoprotein fractions of SEA, (2) preparation of immune sera and antibodies of SEA and its glycoprotein fractions, and (3) establishment of solid phase ELISA method in the determination of SEA and its glycoprotein fractions. It was found that SEA hydrophobic glycoprotein has strong immune activity and its corresponding antibody is most sensitive and specific. When anti-hydrophobic glycoprotein antibody was used to determine crude SEA, glycoprotein fraction and hydrophobic glycoprotein fraction, the quantity detected could be as low as 15ng/ml, 6ng/ml and 2ng/ml respectively. The use of anti-SEA hydrophobic glycoprotein antibody for SEA determination is a valuable method.更多还原