【摘要】 培养的新生BALB/c小鼠搏动心肌细胞感染柯萨奇B-2病毒4h加同种小鼠腹腔接种该病毒7d后的脾细胞共同孵育1d,后者能轻度加重被感染心肌细胞的超微结构改变,包括局部细胞膜边界模糊;细胞质内呈较为严重的胞浆浓缩、不均一和空隙形成及闰盘解离等。小鼠心肌细胞感染柯萨奇B-2病毒3d,其超微结构改变较感染4h者严重。实验结果提示T细胞介导免疫及病毒本身在急性病毒性心肌炎的发病中都起重要作用。更多还原

【Abstract】 Spleen cells from BALB/c mice which had received i. p. innoculation of Ooxsackie B-2 virus 7 days previously, were added to cultured beating myocytes of syngenetic neonate mice infected 4 hours before with the same virus, and cultured together for 1 day. Ultrastructural changes of infected myocytes were found to be slightly aggravated by the spleen cells. The myocytes showed local blurring of cell bounds, marked concentration and inhomogeneity of the cytoplasm resulting in space, formation around it, and dilatation as well as disintegration of the intercalated discs. The Ultrastructural changes were more prominent in mouse myocytes infected with Ooxsackie B-2 virus for 3 days than in those for 4 hours. Our experimental results suggest that T lymphocyte-mediated immunity and the virus itself both play important roles in the pathogenesis of acute viral myocarditis.更多还原