【摘要】 本实验用妊娠13～15d的小鼠胎肝细胞血浆凝块培养法测定人尿提取物红细胞生成素(EPO)生物活性。实验证明,在相同细胞浓度的培养体系中,红系祖细胞(E-CFU。)产率与EPO浓度(在5～100mu/ml范围内)呈高度正相关。以EPO(7A)为参照标准,用E-CFU。方法测得EPO(850323)活性与缺氧多血小鼠标准的体内分析方法所获得结果一致。提取工艺路线具有较好的重复性。冻干EPO制品溶解后保存于—20℃,6周内活性不变。更多还原

【Abstract】 Erythroid colony forming unit in plasma clots (E-CFU0) has been used to determine erytliropoietin (EPO) activity in human urinary extracts. Fetal mouse liver at 13-15th d of gestation was used in this study. In the liver there are many erythroid colony forming cells that are highly sensitive to EPO. Taking EPO (7A) as a reference standard, we found that the E-OFU0 number is proportional to the EPO concentration in the medium within a dose range of 5-100 mU/ml. The EPO activity of the extract (EPO 850323) determined by this method corresponded well with results using the exhypoxic polyoythemio mouse assay. The EPO activity of extracts in different batches using the same technique varied. EPO activity of the preparation (EPO 850323) dissolved in EPMI 1640 media and then frozen at - 20℃ remained stable for 6 weeks.更多还原