【摘要】 水稻齿叶矮缩病毒(Rice Ragged Stunt Virus,以下简称RRSV)的基因组双链RNA经羟甲基汞变性处理后,能在兔网状红细胞体外翻译体系中有效地指导蛋白质生物合成。利用该病毒的抗血清免疫沉淀体外翻译产物,结合SDS-聚丙烯酰胺凝胶电泳分析,证明RRSV基因组RNA体外翻译的主要产物有十种左右,其中八种能被该病毒抗血清专一沉淀。用纯化的水稻齿叶矮缩病毒直接作蛋白质电泳分析,证明上述八种体外翻译产物与该病毒结构蛋白的分子量相当。Schiff试剂染色实验未能检测出糖蛋白。更多还原

【Abstract】 10 segments from the ds-RNA of RRSV were denatured with heat, dimethylsulfone (DMSO) and hydroxymethyl mercury. Only the segments denatured with hydroxymethyl mercury could be translated in vitro in rabbit reticulocyte cell-free systems, while the undenatured and heat-or DMSO-denatured segments were unable to direct protein synthesis. The translation products were analysed by SDS-PAGE and 10 poly-peptides with molecular weights of 120, 90, 88, 76, 70, 63, 43, 39, 33 and 31 Kd were revealed. Immunoprecipitation tests of the translation products with the antiserum against purified RRSV demonstrated that 8 polypoptides with molecular weights of 120, 94, 70, 60, 49, 43, 39 and 33Kd could be precipitated and concentrated. The SDS-PAGE of partially purified RRSV preparations showed 8 polypoptides with molecular weights of 125, 97, 66, 64, 48, 43, 38, 32 Kd and some minor bands. These results indicated that the genomes of RRSV may possibly encode 8 structural proteins and 2 or 3 nonstructural proteins. Investigations on the occurrence of post-translational processing and of the nature of the polypeptides encoded by each individual segment of RRSV ds-RNA are being continued.更多还原