【摘要】 本文主要阐述关于牛的体外受精的一般操作过程:卵母细胞的采集、体外成熟培养、授精前精子获能的预处理,体外授精及受精卵的培育。试验结果表明对冷冻精液进行离心清洗可以提高体外受精率,其中无清洗或1次清洗后的精子受精率均为0;2次或3次清洗的精子受精率分别达36.1%和43.65。更多还原

【Abstract】 This paper expounded the operative process of fertilization in vitro:Collection of oocytes,Culture in vitro,Pretreatment of sperm capacitation before insemination, insemination in vitro and embryogenic culture in cattle.The experiment showed that washing frozen semen may increased the fertility rate in vitro,the fertility rates of unwashed or washed 1 time are 0;the fertility rates of washed 2 or 3 times are 36.1% and 43.6% respectively。更多还原