【摘要】 用 SDS 聚丙烯酰胺凝胶电泳分离鸡传染性支气管炎病毒的结构多肽,可以分得数十根条带,其中四条是主要的多肽。比较多肽图谱的差别,显示了病毒蛋白结构上的差别。结果表明,鸡传染性支气管炎病毒在密度梯度离心时,两个主要区带的病毒粒子在蛋白结构上是一致的。而该病毒的国内流行株系与现在通常作为疫苗使用的国外弱毒株系之间则表现较大区别,显示了它们在蛋白结构上有较大差异。提示目前在国内广泛使用的鸡传染性支气管炎疫苗的免疫保护作用是有缺陷的。更多还原

【Abstract】 Isolation of the structural polypeptides of Avian infectious bronchitis virus with SDS polyacrylamide gel electrophoresis showed the difference of viral protein.Comparisons of the structural polypeptides of IBV from different peak of sucrose density gradients showed that they have similar polypeptide patterns. Examination of structural polypeptides of various IBV strains have produced results indicating that there are quite difference between those avirulent strains used as vaccines and epidemic strain of Shanghai.So the common IBV vaccines such as H120,H52 cannot act well,because of the defect of immune protection.更多还原