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木槿、栀子、倒挂金钟愈伤组织的诱导和再生植株的形成

Induction of Callus and Regeneration of Plantlets Originated From Hibiscus Syriaeus Gardenia Jasminoides and Fuchsia Hybrida

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【作者】 李世承; 赵彦芳; 邹杰; 李晶;

【Author】 Li Shicheng Zhao Yanfang Zou Jie Li Jing Department of Biology, Liaoning University

【机构】 辽宁大学生物系; 辽大花房;

【摘要】 本文报道了木槿,栀子、倒挂金钟外植体经组织培养形成了再生植株的过程,在以1/2MS+6-BA?+IAA0.1(或IBA0.1或2.4-D0.1~0.5)+GA0.5+LH100为培养基时,培养木槿叶外植体,经3个月左右,可获得再生植株,在以1/2MS+6-BA3+NAA0.1~0.2+LH100为培养基培养栀子、倒挂金钟茎段外植体时,经2~3个月培养,均可获得茎段外植体的再生苗。当小苗长至1.5~2厘米高时,截取幼苗转移到1/2MS+6-BA0.5(或KT0.5)+NAA0.5+IBA0.5+LH100或1/2MS+NAA5+LH100的生根培养基上,经20天左右的培养,均能分化出根来,形成完整植株。

【Abstract】 This paper reported the regeneration of plantlets through tissue cultivation in vitro from Hibiscus syriacus , Gardenia jasminoides and Fuchsia bybrida.Being cultivated for 3-4 months in the medium containing(1/2)MS+6--BA3+IAA0.1 (or IBA0.1, or 2.4-D0.1~0.5) +GA0.5, The regenerating plantlets were obtained from the leaves of Hibiscus syriacus in vitro Being cultivated for 2-3 months in the medium containing(1/2)MS+NAA0.1~0.2+6-BA3, the regenerating pantlets were obtained from both the stem segments of Gardenia jasminoides and Fuchsia hybrida in vitro.Being 1.5-2 cm high, the plantlets were cut off and then transplanted in the rooting medium containing (1/2)MS+NAA0.5+IBA0.5+KT0.5(or 6-BA0.5)+LH100, Being cultivated for 15-20 days, the roots could be differentiated and the plants developed fully.

  • 【文献出处】 辽宁大学学报(自然科学版) ,