【摘要】 本文研究了雄性小白鼠在青春期前阉割对其成年后脑中核酸、某些氨基酸及环一磷酸腺苷含量的影响。发现阉割小鼠脑中GABA、谷氨酸及RNA-P含量(按单位脑组织湿重计)分别为2.68±0.04μg/g、5.8±0.17μg/g、13.5±0.87mg/100g,比假手术同龄对照动物差异极其显著(P<0.01)。而谷氨酰胺,DNA及cAMP含量,则两者无显著差异。阉割后肌注丙酸睾丸酮可使脑中核酸、氨基酸含量保持正常,仅注油溶液无此代偿效应。并讨论了睾丸激素影响脑功能发育的可能机理。更多还原

【Abstract】 The effects of prepuberal castration on the amout of nucleic acid, some amino acids and cyclic AMP of male mouse brain were studied. Mice castrated at 40 days of life were killed at the age of 100 days. The amounts of nucleic acid, amino acid, cyclic AMP in the castrated and control male mouse brains had been determined by means of modified Schneider’s method, paper chromatography and competitive protein binding assay respectively. The amounts of GABA, glutamate and RNA of castrated mouse brains (2.68±0.04μMol/g fresh brain tissue, 5.8± 0.17 μMol/g fresh brain tissue, 13.5±0.87 mg/100g fresh brain tissue, respectively) were significantly less than that of the control(3.38±0.07, 8.8±0.28, 20.1±0.14, respectively). But the amounts of glutamine, DNA and cyclic AMP in castrated and control mouse brains showed no signi- ficant difference. After intramuscular injection of testosterone propio- nate into the castrated mouse, the amounts of GABA, glutamate and RNA in the brain showed no difference with control. The possible me- chanism of the effect of male sex hormone upon the nervous system is discussed.更多还原