【摘要】 本文详细描述了一种测定脱氧核糖核酸(简称DNA)中鸟嘌吟(简称G)加胞嘧啶(简称C)百分含量的方法。将DNA样品分装于数支试管内,一起置于JY-501型超级恒温器中,加热至不同的预选温度并恒温10min,每次恒温后取出一支样品立即冰浴。然后测定其在260nm的吸收值。绘出温度与相对增色值之间的关系曲线,确定DNA的变性温度(Tm)并计算GC百分含量。对DNA的变性过程进行了电泳检查。本方法结果与前人报告的结果基本相符。更多还原

【Abstract】 A simple method was described in detail for determining the percentage of guanine ( G ) plus cytosine ( C ) in Deoxyribonucleic Acid by using 751-G Spectrophotometer which did not have a Constant Temperature Water Circulation Device. The DMA samples were heated and equilibrated about 10 min at each selected temperature by JY-501 Constant Temperature Instrument. After heating, the optical desities of DNA samples at each temperature were read at 260 nm and divided by the value at 30℃.It allowed to determine G + C percentages of more than one DNA samples simultaneously. Values obtained by this method for the percentage of G + C gave a close agreement with the values previously observed.更多还原