【摘要】 本研究建立了小鼠胎心器官培养方法,培养的心脏可存活和自主性搏动达27±2.3(SD)天,并详细观察了培养不同时间小鼠胎心心肌细胞发育的超微结构变化特征。更多还原

【Abstract】 A technique has been developed for maintaining isolated hearts from 16-20 days fetal mice in organ culture. In the study, the hearts cultured lived and beat spontaneously for 27 2.3 (SD) days. At intervals of 1-3 days, the hearts showed normal ultrastructure and growing and proliferating morphology, most myocardial cells remained normal ultrastructure for 14 days. The fetal mouse hearts in organ culture may serve as useful model for studying in vitro the chronic effects of experimental agents on cardiac growth, function and morphology.更多还原