【摘要】 本文采用辣根过氧化物酶标记伴刀豆球蛋白A(Con A-HRP)法来研究人和犬、大小鼠消化管各段Con A受体的分布。结果表明:各段消化管粘膜上皮细胞都具有不同程度的阳性反应。除胃粘膜上皮细胞的核上区为阴性外,其他各段均以核上区反应最强。杯状细胞除人和犬的小肠及大小鼠的大肠阴性反应外,均有程度不等的阳性反应。胃底腺内壁细胞和主细胞的反应有明显的种系差异,人和犬二种细胞都为阳性反应,而大小鼠只有主细胞有阳性反应。肠腺阳性反应。神经元有较强的反应。平滑肌有不同强度的反应,人、犬比大小鼠强。由此提示消化管是一个高度异质性的细胞群体,并有种系差异。更多还原

【Abstract】 The distribution of Con A receptor in gastrointestinal tract of human, rat,mouse and dog was investigated using horseradish peroxidase labeled Con A staining method. It has been shown that Con A receptors were existed, more or less, in mucosal epithelia taken from different segments of tract. The most intense staining was noticed in supranuclear region of epithelial cells, except for gastric mucosa, which had a negative supranuclear region. The staining character of goblet cells was heterogenous: negative staining was verified in the goblet cells of small intestine from human and dog, and large intestine from rat and mouse, while the goblet cells in other place all showed more or less positive reaction. There was significant species difference of staining reaction in chief cell and parietal cell of gastric glands: both cells showed positive staining in human and dog, while only chief cells showed positive reaction in rat and mouse. Besides, Con A receptors were also found in intestinal glands, neurons of plexus of Auerbach and Meissner, smooth muscle. In a word, a highly heteroglneous cell populations were in gastrointestinal tract and possessed species difference.更多还原