【摘要】 本实验通过外源性3′,5′环腺苷酸（cAMP）对人胃腺癌SGC-7901细胞系的连续作用,观察到癌细胞的增殖受到明显抑制的同时,膜表面环腺苷酸-磷酸二酶（cAMP-PDEase）的活性及Mg⁺⁺-ATPase活性均明显下降,而细胞内cAMP免疫荧光强度则明显增强,进一步证实了细胞内cAMP水平与细胞的生长、分化,及膜表面ATPase、cAMP-PDEase的活性有密切的关系。更多还原

【Abstract】 The experiment was carried out on cultured human stomach glandular carcinoma （SGC-7901 cell line）. We previously reported the cytochemical changes of （Na⁺-K⁺）-ATPase activity and the changes of microvilli. We here studied the relationship among the cAMP, cAMP-PDEase, Mg⁺⁺-ATPase activity, the cell growth and differentiation. Cells were exposed to Immol/L cAMP together with 1 mmol/L theophylline. We showed cAMP specific fluorescence by immunocytochemical method, Mg⁺⁺-ATPase and cAMP-PDEase activity by cytochemical methods. We found that cAMP with theophylline not only inhibited the growth of these SGC-7901 cells as shown by our previous result, but also decreased the activity of Mg⁺⁺-ATPase and 3’, 5’-cyclic monophosphate phosphodiesterase. The result indicates that exogenous cAMP can inhibit 3’, 5’-cAMP-PDEase and Mg⁺⁺-ATPase activity and increase cAMP level in cell, and cAMP regulated the growth and differentiation of SGC-7901 cell line更多还原