【摘要】 <正> 我们通过测定葡萄糖异构酶的CD光谱了解其结构与温度的关系。该酶催化葡萄糖转化为果糖,果糖的甜度高于葡萄糖和蔗糖,是优良的蔗糖代用品。该酶的研究引起了世界各国科学家的广泛注意。美、日、苏及丹麦等国做了多方面的研究并取得了突破性的成绩。目前国外已大规模地使用葡萄糖异构酶生产果糖。但对其作用方式、结更多还原

【Abstract】 The optimum temperature of glucose isomerase activity is 70—80℃, We measured the activity of the enzyme treated with different temperature to investigate the relation between structure and temperature of the enzyme. The activity of the glucose isomerase was 6.8u/ml, 5.9u/ml and 4.9u/ml by treated 5 min at the temperature of 50℃,55℃,60℃ respectively. The activity of untreated one was 4.1u/ml. The dichroism spectra of glucose isomerase were detected at different temperature. The degree of α-helix was 38% at room temperature. The degree of helix decrease about 2% with the increasing temperature of 10℃ compared with roomtemperature.更多还原