【摘要】 作者用SDS-PAGE和Western印迹分析了三株钩体全细胞蛋白电泳图谱及McAb识别的抗原分子。致病性问号钩端螺旋体017株、606株蛋白电泳图谱平均相似性为90％,与非致病性双曲钩端螺旋体patoc Ⅰ株相比,分别为32％,31％;与McAb LB₁ 识别的抗原图谱相比,017株与606株基本一致,与patoc Ⅰ株均完全不同。提示该分析方法在区分致病性与非致病性钩体上具有一定意义。更多还原

【Abstract】 The electrophoretic whole-cell proteinprofiles and McAb recognized moleculesof pathogenic Leptospira interrogans serovarLai strain 017, L. interrogans serovar au-tumnalis strain 606 and nonpathogenic L.biflexa serovar patoc strain patoc I wereanalysed by SDS-PAGE and Western blottechniques. Whole-cell proteins of strains were allseparated into at least 40 distinguishablebands on 10% gel and, the protein profilesof two pathogenic strains were very similar（average similarity = 90%）, but were widelydifferent from those of nonpathogenicstrain patoc I （average similarity = 32% and31%）. After being transferred from gels tonitrocellulose filters （0.45μm pore size）,about 10 to 12 distinct bands of each strainproteins were revealed as antigens bystaining with McAb LB₁(IgG_2b) againstL. interrogans serova Lai strain 017 inELISA. The antigenic profiles of bothpathogenic strains were basically identical,but were different at all from that ofnonpathogenic strain with the exceptionof one common molecular of 45000. Our results indicated that characteri-zation of the whole-cell protein profilesand McAb recognized antigen moleculescan reflect the homologous relationshipbetween leptospiral strains, and it may bevaluable in the indenfication of pathogenicand nonpathogenic leptospires.更多还原