【摘要】 作者对核糖体现有的制备方法进行了部分改进,从黄疸出血群赖型017株钩体中成功地制备了核糖体提取物。该提取物经SPA-ELISA 证实具有免疫原性,对40只受试豚鼠的保护试验结果表明,核糖体提取物对同型毒株的攻击具有明显的保护作用;结果还证明核糖体提取物能诱导机体的体液免疫反应。更多还原

【Abstract】 Ribosomal extract was isolated from L.Interrogans Serogroup icterohaemorrhagiaeSerovar Lai Strain 017 by ultrasoni disrup-tion of the cell wall and differential centri-fugation.Chemical analysis indicated thatprotein accounted for 33%,RNA 60% andDNA 1% of the ribosomal composition.Elec-tron microscopic observation with negativestain showed the ribosomal preparationcontained ribosomal particles. The immunological protective animal testshowed that the ribosomal extract had theprotective function against attack of autolo-gous virulent strain of leptospires.The pro-tective rate was 85±5.6% In the presenceof ribosomal antiserum and complement,there was marked killing of aotologous lep-tospires.The antiserum had opsonophago-cytic activity and enhanced phagocytosisof aotologous strain of leptospires byguinea-pig macrophages.更多还原