【摘要】 利用八倍体小黑麦‘h739’护颖分化期幼穗作外植体,成功地建立了愈伤组织诱导和再生程序,并获得了长期保持胚性的无性系。经446天继代培养后,在HC-0、HC-4和HC-5三个无性系再生植株中观察到广泛的形态变异,包括育性、株高、抽穗期、穗形和穗颈毛等。在SC₁代中这些变异除抽穗期外均有较坚实的遗传基础。SC₂代的表现表明,在SC₁代发生的是隐性突变或纯合突变更长时间（852天）继代后,无性系变异更丰富,但育性明显下降,有害突变增加,所以在体细胞无性系变异中也有一个适宜的继代时间问题。更多还原

【Abstract】 Efficient callus induction and regeneration procedure were established using growing tip of octoploid triticale ’h739’ at glume differentiation stage as explants.Embryonic somaclones were selected.Extensive somaclonal morphological variation was observed in regenerants of HC-4,HC-5 and HC-0 lines after 446 days’ subculture.Variant characters included fertility,height,heading date,spike shape,hairy neck.Evaluation of the SC2 progeny indicated strong genetic basis for these variants except heading date.Segregation data suggested that homozygous and recessive mutations had occurred in SCl generation.More variations after 852 days’ subculture were observed.However,grain fertility showed a decline and adverse mutations increased.It is seems that the influence of subculture times was significant in the somaclonal variation.更多还原