【摘要】 本文讨论了将光质应用于一品红(Euphorbia pulcherrima)大规模细胞繁殖及形态发生调控的可行性,并测定了一些形态及生化指标作为这种调控的参数。 以一品红试管苗的叶片作为外植体,置于MS培养基中并分别培养于白(W)、红(R)、黄(Y)、蓝(B)、绿(G)色光及黑暗(D)下,一个月后进行测定。蓝光和黄光明显促进出苗。而愈伤组织形成的情况则正相反,红光利于愈伤组织的形成,黄光效果最差。过氧化物酶、过氧化氢酶活力及蛋白质、RNA、碳水化合物的含量与愈伤组织形成有较为一致的关系,但与分化不直接相关。 实验中还测定了叶绿素a、叶绿素b及类胡萝卜素的含量。白光与蓝光均能促进叶绿素及类胡萝卜素的合成,但蓝光中chl b/chl a的比率较高。此外,本文尚涉及暗处理中的晶质化现象(vitrification)及其成因。更多还原

【Abstract】 The recent paper deals with the feasibility of light quality regulation on large-scale plant cell proliferation and morphogenesis of Euphorbia pulcherrima. Some morphological and biochemical changes were measured as the parameters of the regulation.Leaves from single genotype of Euphorbia pulcherrima were cultured in white, red, yellow, green, blue lights and in dark respectively. Blue and yellow lights stimulated shooting significantly while callus growth was quite the opposite. Red light was most effective to callus formation and yellow the least effective. The activities of peroxidase, hydrogen peroxidase and contents of protein, RNA, carbohydrate were relatively consistent with the ability of callus formation but not differentiation.Contents of chlorophyll a, chlorophyll b and carotenoids were also determined. White and blue lights increased both chlorophyll and carotenoids contents while the ratio of Ch1 b/Ch1 a was much higher in blue light.The vitrification phenomena in dark treatment was also discussed.更多还原